Title: | Overexpression of delta-12 desaturase in the yeast Schwanniomyces occidentalis enhances the production of linoleic acid. |
Author(s): | Lamers, W.L. ; Visscher, B. ; Weusthuis, R.A. ; Francke, C. ; Wijffels, R.H. ; Lokman, B.C. |
Publication year: | 2019 |
Source: | Bioresource Technology, vol. 289, (2019), pp. 121672 |
ISSN: | 0960-8524 |
DOI: | https://doi.org/10.1016/j.biortech.2019.121672 |
Publication type: | Article / Letter to editor |
Please use this identifier to cite or link to this item : https://hdl.handle.net/20.500.12470/2296 ![]() |
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Lectorate : | Biobased Innovations |
Journal title : | Bioresource Technology |
Volume : | vol. 289 |
Page start : | p.121672 |
Abstract: |
The oleaginous yeast Schwanniomyces occidentalis was previously isolated because of its excellent suitability to convert lignocellulosic hydrolysates into triacyl glycerides: it is able to use a broad range of sugars and is able to tolerate high concentrations of lignocellulosic hydrolysate inhibitors. Compared to other oleaginous yeasts S. occidentalis however produces a low content of unsaturated fatty acids. We show here that the linoleic acid content can be significantly improved by (over)expression Δ12-desaturases derived from S. occidentalis and Fusarium moniliforme. Expression was stable for the homologous expression but decreased during heterologous expression. Both homologous and heterologous expression of mCherry-Δ12-desaturase led to a 4-fold increase in linoleic acid from 0.02 g/g biomass to 0.08 g/g biomass resulting in the production of 2.23 g/L and 2.05 g/L of linoleic acid.
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